研究業績

論文

1999年以前

  1. Kobashigawa Y, Sakurai M, Nitta K.
    Effect of hydrostatic pressure on unfolding of alpha-lactalbumin: volumetric equivalence of the molten globule and unfolded state.
    Protein Sci. 8,2765-2772 (1999).
  2. Kobayashi H, Kato J, Morioka H, Stewart JD, Ohtsuka E.
    Tryptophan H33 plays an important role in pyrimidine (6-4) pyrimidone photoproduct binding by a high-affinity antibody.
    Protein Eng. 12, 879-884 (1999).
  3. Morioka H, Kobayashi H, Kurihara M, Kato J, Komatsu Y, Sato K, Nobuoka K, Kato K, Torizawa T, Shimada I, Satow Y, Stewart JD, Matsunaga T, Nikaido O, Ohtsuka E.
    Analysis of molecular recognition of antibodies specific for UV-damaged DNA.
    Photomed. Photobiol. 21, 7-8 (1999).
  4. Torizawa T, Kato K, Kato J, Kobayashi H, Komatsu Y, Morioka H, Nikaido O, Ohtsuka E, Shimada I.
    Conformational multiplicity of the antibody combining site of a monoclonal antibody specific for a (6-4) photoproduct.
    J. Mol. Biol. 290, 731-740 (1999).
  5. Ohtsuka E, Kobayashi H, Morioka H, Sato K, Komatsu Y, Nikaido O, Satow Y, Shimada I.
    Interactions of high affinity anti (6-4) photoproduct antibody fragments with damaged DNA.
    Nucleosides & Nucleotides 18, 1321-1322 (1999).
  6. Kobayashi H, Sato K, Komatsu Y, Morioka H, Stewart JD, Tsurimoto T, Ohtsuka E.
    Effects of a high-affinity antibody fragment on DNA polymerase reactions near a (6-4) photoproduct site.
    Photochem. Photobiol. 69, 226-230 (1999).
  7. Kobayashi H, Morioka H, Tobisawa K, Torizawa T, Kato K, Shimada I, Nikaido O, Stewart JD, Ohtsuka E.
    Probing the interaction between a high-affinity single-chain Fv and pyrimidine (6-4) pyrimidone photodimer by site-directed mutagenesis.
    Biochemistry 38, 532-539 (1998).
  8. Kobayashi H, Morioka H, Nikaido O, Stewart JD, Ohtsuka E.
    The role of surface lysines in pyrimidine (6-4) pyrimidone photoproduct binding by a high-affinity antibody.
    Protein Eng. 11, 1089-1092 (1998).
  9. Kobayashi T, Takeuchi S, Saijo M, Nakatsu Y, Morioka H, Otsuka E, Wakasugi M, Nikaido O, Tanaka K.
    Mutational analysis of a function of xeroderma pigmentosum group A (XPA) protein in strand specific DNA repair.
    Nucleic Acids Res. 26, 4662-4668 (1998).
  10. Oku T, Ikeda S, Sasaki H, Fukuda K, Morioka H, Ohtsuka E, Yoshikawa H, Tsurimoto, T.
    Functional sites of human PCNA which interact with p21 (Cip1/Waf1), DNA polymerase d and replication factor C.
    Genes to Cells 3, 357-369 (1998).
  11. Torizawa T, Kato K, Kimura Y, Asada T, Kobayashi H, Komatsu Y, Morioka H, Nikaido O, Ohtsuka E, Shimada I.
    31P NMR study of the interactions between oligodeoxynucleotides containing (6-4) photoproduct and Fab fragments of monoclonal antibodies specific for (6-4) photoproduct.
    FEBS Lett. 429, 157-161, (1998).
  12. Morioka H, Miura H, Kobayashi H, Koizumi T, Fujii K, Asano K, Matsunaga T, Nikaido O, Stewart JD, Ohtsuka E.
    Antibodies specific for (6-4) DNA photoproducts: Cloning, antibody modeling and construction of a single-chain Fv derivative.
    Biochim. Biophys. Acta 1385, 17-32 (1998).
  13. Kai M, Todo T, Wada M, Ryo H, Masutani C, Kobayashi H, Morioka H, Ohtsuka E, Hanaoka F, Sakaguchi K.
    A new drosophila ultraviolet light-damaged DNA recognition endonuclease that selectively nicks a (6-4) photoproduct site.
    Biochim. Biophys. Acta 1397, 180-188 (1998).
  14. Kobayashi H, Morioka H, Torizawa T, Kato K, Shimada I, Nikaido O, Ohtsuka E.
    Specificities and rates of binding of anti-(6-4) photoproduct antibody fragments to synthetic thymine photoproducts.
    J. Biochem. 123, 182-188 (1998).
  15. Kimura S, Kai M, Kobayashi H, Suzuki A, Morioka H, Ohtsuka E, Sakaguchi K.
    A Structure-specific endonuclease from cauliflower (Brassica oleracea var. botrytis) inflorescence.
    Nucleic Acids Res. 25, 4970-4976 (1997).
  16. Todo T., Kim S-T, Hitomi K, Otoshi E, Inui T, Morioka H, Kobayashi H, Ohtsuka E, Toh H, Ikenaga, M.
    Flavin adenine dinucleotide as a choromophore of the Xenopus (6-4)photolyase.
    Nucleic Acids Res. 25, 764-768 (1997).
  17. Shimizu M, Morioka H, Inoue H, Ohtsuka E.
    Triplex-mediated cleavage of DNA by 1,10-phenanthroline-linked 2'-O-methyl RNA.
    FEBS Lett. 384, 207-210 (1996).
  18. Fukuda K, Morioka H, Imajou S, Ikeda S, Ohtsuka E, Tsurimoto T.
    Structure-function relationship of the eukaryotic DNA replication factor, Proliferating Cell Nuclear Antigen.
    J. Biol. Chem. 270, 22527-22534 (1995).
  19. Nakae J, Morioka H, Ohtsuka E, Fujieda K.
    Replacements of leucine 87 in human insulin receptor alter affinity for insulin.
    J. Biol. Chem. 270, 22017-22022 (1995).
  20. Iwai S, Maeda M, Shimada Y, Hori N, Murata T, Morioka H, Ohtsuka E.
    Endonuclease V from bacteriophage T4 interacts with its substrate in the minor groove.
    Biochemistry 33, 5581-5588 (1994).
  21. Gohda K, Itoh T, Hiramatsu Y, Tomita K, Nishikawa S, Uesugi S, Morioka H, Ohtsuka E, Ikehara M, Hakoshima T.
    Crystal structure of RNase T1 (Y45W) complexed with 3'AMP and GflpA.
    J. Biochem. 114, 842-848 (1993).
  22. Yu K, Morioka H, Fritze LMS, Beeler DL, Jackman RW, Rosenberg RD.
    Transcriptional regulation of the thrombomodulin gene.
    J. Biol. Chem. 267, 23237-23247 (1992).
  23. Hakoshima T, Itoh T, Tomita K, Gohda K, Nishikawa S, Morioka H, Uesugi S, Ohtsuka E, Ikehara M.
    Three-dimensional structure of a mutant ribonuclease T1 (Y45W) complexed with non-cognizable ribonucleotide, 2'AMP, and its comparison with a specific complex with 2'GMP.
    J. Mol. Biol. 223, 1013-1028 (1992).
  24. Itoh T, Tomita K, Hakoshima T, Hiroaki H, Uesugi S, Nishikawa S, Amisaki T, Morioka H, Ohtsuka E, Ikehara, M.
    Conformation of 2'GMP bound to a mutant ribonuclease T1 (Y45W) determined by X-ray diffraction and NMR methods.
    J. Biochem. 110, 677-680 (1991).
  25. Hakoshima T, Tanaka M, Itoh T, Tomita K, Amisaki T, Nishikawa S, Morioka H, Uesugi S, Ohtsuka E, Ikehara M.
    Hydrophobic effects on protein/nucleic acid interaction: enhancement of substrate binding by mutating tyrosine 45 to tryptophan in ribonuclease T1.
    Protein Eng. 4, 793-799 (1991).
  26. Hakoshima T, Itoh T, Gohda K, Tomita K, Uesugi S, Nishikawa S, Morioka H, Ohtsuka E, Ikehara M.
    Non-cognizable ribonucleotide, 2'AMP, binds to a mutant ribonuclease T1 (Y45W) at a new base-binding site but not at the guanine-recognition site.
    FEBS Lett. 290, 216-220 (1991).
  27. Ueda H, Maruyama H, Doi M, Inoue M, Ishida T, Morioka H, Tanaka T, Nishikawa S, Uesugi S.
    Expression of a synthetic gene for human cap binding protein (human IF-4E) in Escherichia coli and fluorescence studies on interaction with mRNA cap structure analogues.
    J. Biochem. 109, 882-889 (1991).
  28. Ueda H, Iyo H, Doi M, Inoue M, Ishida T, Morioka H, Tanaka T, Nishikawa S, Uesugi S.
    Combination of Trp and Glu residues for recognition of mRNA cap structure. Analysis of m7G base recognition site of human cap binding protein (IF-4E) by site-directed mutagenesis.
    FEBS Lett. 280, 207-210 (1991).
  29. Hakoshima T, Itoh T, Tomita K, Nishikawa S, Morioka H, Uesugi S, Ohtsuka E, Ikehara M.
    Crystallization and preliminary X-ray investigation of non-specific complexes of a mutant ribonuclease T1 (Y45W) with 2'AMP and 2'UMP.
    J. Mol. Biol. 216, 497-499 (1990).
  30. Hakoshima T, Oka K, Toda S, Tanaka M, Gohda K, Higo T, Itoh T, Minami H, Tomita K, Nishikawa S, Morioka H, Imura J, Uesugi S, Ohtsuka E, Ikehara, M.
    Crystallographic characterization of wild-type and mutant ribonuclease T1 complexes with several ribonucleotides.
    J. Biochem. 108, 695-698 (1990).
  31. Fujimura T, Tanaka T, Ohara K, Morioka H, Uesugi S, Ikehara M, Nishikawa S.
    Secretion of recombinant ribonuclease T1 into the periplasmic space of Escherichia coli with the aid of the signal peptide of alkaline phosphatase.
    FEBS Lett. 265, 71-74 (1990).
  32. Nishikawa S, Adiwinata J, Morioka H, Fujimura T, Tanaka T, Uesugi S, Hakoshima T, Tomita K, Nakagawa S, Ikehara M.
    A thermoresistant mutant of ribonuclease T1 having three disulfide bonds.
    Protein Eng. 3, 443-448 (1990).
  33. Hakoshima T, Toda S, Sugio S, Tomita K, Nishikawa S, Morioka H, Fuchimura K, Kimura T, Uesugi S, Ohtsuka E, Ikehara M.
    Conformational properties of the guanine-binding site of ribonuclease T1 inferred from the X-ray structure and protein engineering.
    Protein Eng. 2, 55-61 (1988).
  34. Nishikawa S, Morioka H, Kimura T, Ueda Y, Tanaka T, Uesugi S, Hakoshima T, Tomita K, Ohtsuka E, Ikehara M.
    Increase in nucleolytic activity of ribonuclease T1 by substitution of tryptophan 45 for tyrosine 45.
    Eur. J. Biochem.173, 389-394 (1988).
  35. Nishikawa S, Kimura T, Morioka H, Uesugi S, Hakoshima T, Tomita K, Ohtsuka E, Ikehara M.
    Glu 46 of ribonuclease T1 is an essential residue for the recognition of guanine base.
    Biochem. Biophys. Res. Comm. 150, 68-74 (1987).
  36. Nishikawa S, Morioka H, Kim HJ, Tanaka T, Uesugi S, Hakoshima T, Tomita K, Ohtsuka E, Ikehara M.
    Two histidine residues are essential for ribonuclease T1 activity as is the case for ribonuclease A.
    Biochemistry 26, 8620-8624 (1987).
  37. Ikehara M, Nishikawa S, Morioka H, Kim HJ, Fuchimura K, Kimura T, Adiwinata J, Tanaka T, Ohtsuka E, Uesugi S.
    Mechanism of hydrolysis of phosphodiesters with ribonuclease T1.
    Pure & Appl. Chem. 59, 965-968 (1987).
  38. Nishikawa S, Morioka H, Fuchimura K, Tanaka T, Uesugi S, Ohtsuka E, Ikehara M.
    Modification of Glu 58, an amino acid of the active center of ribonuclease T1, to Gln and Asp.
    Biochem. Biophys. Res. Comm. 138, 789-794 (1986).
  39. Ikehara M, Ohtsuka E, Tokunaga T, Nishikawa S, Uesugi S, Tanaka T, Aoyama Y, Kikyodani S, Fujimoto K, Yanase K, Fuchimura K, Morioka H.
    Inquiries into the structure-function relationship of ribonuclease T1 using chemically synthesized coding sequences.
    Proc. Natl. Acad. Sci. USA 83, 4695-4699 (1986).
  40. Ohtsuka E, Matsugi J, Yamane A, Morioka H, Ikehara M.
    Studies on transfer ribonucleic acids (tRNA) and related compounds, XLIX. Solid-phase synthesis of nonadecaribonucleotide corresponding to the 5'-half of the formylmethionine tRNA deficient in D-loop and stem region.
    Chem. Pharm. Bull. 33, 4152-4159 (1985).
  41. Doi T, Morioka H, Matsugi J, Ohtsuka E, Ikehara M.
    Modification of the amino acid acceptor stem of E.coli tRNAMet by ligation of chemically synthesized ribooligonucleotides.
    FEBS Lett. 190, 125-128 (1985).
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